We are interested in how the human cell cycle is regulated, especially in the context of stress to cells. The cell cycle is driven by periodic fluctuations in protein levels and enzyme activities. These fluctuations are coupled together, in analogy to a set of falling domino bricks. When a set of domino bricks are falling, the movement can only go in one direction and once started, it is bound to continue until the last brick has fallen. However, if you artificially block the movement, for example by removing a brick that is about to fall, you need to actively push on the next brick to re-start the movement. Similarly, the cell cycle is interrupted by the presence of DNA-damage. When the damage is repaired, cell cycle proteins that normally are redundant become essential to re-start cell cycle progression. At the same time, … See more …
DNA Replication Determines Timing of Mitosis by Restricting CDK1 and PLK1 Activation.
Lemmens B, Hegarat N, Akopyan K, Sala-Gaston J, Bartek J, Hochegger H, Lindqvist A
Mol Cell. 2018 Jul 5
How the cell cycle enforces senescence.
Silva Cascales H, Müllers E, Lindqvist A
Aging (Albany NY). 2017 Oct 30
ATM/Wip1 activities at chromatin control Plk1 re-activation to determine G2 checkpoint duration.
Jaiswal H, Benada J, Müllers E, Akopyan K, Burdova K, Koolmeister T, Helleday T, Medema RH, Macurek L, Lindqvist A
EMBO J. 2017 Jun 12
Residual Cdk1/2 activity after DNA damage promotes senescence.
Müllers E, Silva Cascales H, Burdova K, Macurek L, Lindqvist A
Aging Cell. 2017 Jun
Cell Cycle Dynamics of Proteins and Post-translational Modifications Using Quantitative Immunofluorescence.
Akopyan K, Lindqvist A, Müllers E.
Methods Mol Biol. 2016; 1342:173-83. doi: 10.1007/978-1-4939-2957-3_9.
Spatial Separation of Plk1 Phosphorylation and Activity.
Bruinsma W, Aprelia M, Kool J, Macurek L, Lindqvist A, Medema RH
Front Oncol. 2015 Jun 10;5:132. doi: 10.3389/fonc.2015.00132. eCollection 2015.
Bystander communication and cell cycle decisions after DNA damage.
Jaiswal H, Lindqvist A
Front Genet. 2015 Feb 27;6:63. doi: 10.3389/fgene.2015.00063. eCollection 2015.
Nuclear translocation of Cyclin B1 marks the restriction point for terminal cell cycle exit in G2 phase.
Erik Müllers, Helena Silva Cascales, Himjyot Jaiswal, Adrian T Saurin, Arne Lindqvist
Cell Cycle 2014, September 1
The chromosomal association of the smc5/6 complex depends on cohesion and predicts the level of sister chromatid entanglement.
Jeppsson K, Carlborg KK, Nakato R, Berta DG, Lilienthal I, Kanno T, Lindqvist A, Brink MC, Dantuma NP, Katou Y, Shirahige K, Sjögren C
PLoS Genet. 2014 Oct 16;10(10):e1004680
Picropodophyllin causes mitotic arrest and catastrophe by depolymerizing microtubules via Insulin-like growth factor-1 receptor-independent mechanism.
Waraky A, Akopyan K, Parrow V, Strömberg T, Axelson M, Abrahmsén L, Lindqvist A, Larsson O, Aleem E
Oncotarget. 2014 Sep 30;5(18):8379-92.
Phosphorylation-mediated stabilization of Bora in mitosis coordinates Plx1/Plk1 and Cdk1 oscillations.
Feine O, Hukasova E, Bruinsma W, Freire R, Fainsod A, Gannon J, Mahbubani HM, Lindqvist A, Brandeis M.
Cell Cycle. 2014 Jun 1;13(11):1727-36.