Codes, Scripts, Macros

All scripts on this page are for personal use only. To download script:

  • Press on corresponding “download” link
  • Save zip folder with script on your computer
  • Extract script-files by using any zip archiver (WinZip, WinRAR etc.)
  • Use

Name Software Description
Position list of single cells

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Matlab

MatLab script for making position list of single cells.
The script will scan all images from choosen folder and make a position list of single cells. The script was adapted for 2 different microscope/software system:1. ImageXpress / MetaXpress (Metamorph)2. Leica / LAS MatrixAny other microscope /software can be easily added.The position list will be saved in MatLab folder (root folder).
Analyzing intensities of nucleus and cytoplasm

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Matlab

MatLab script for analyzing intensities of nucleus and cytoplasm.
The script will scan all images from chosen folder and create data file (.xls) with mean and integrated intensities of nucleus and cytoplasm for every channel (channel 1 – DAPI, channel 2 – protein 1, channel 3 – protein 2).
The data file (.xls) will be saved in the destination folder you have chosen.
Analyzing intensities of nucleus, kinetochores and centrosomes

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Matlab

MatLab script for analyzing intensities of nucleus, kinetochores and centrosomes.
The script will scan all images from chosen folder and create data file (.xls) with mean and integrated intensities of nucleus, kinetochores and centrosomes
The data file (.xls) will be saved in the MatLab folder (root folder).
CellCycleKineticsPipeline

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CellProfiler

Pipeline for analyzing intensities of nucleus and cytoplasm. The pipeline will analyze all images from chosen folder and create data file (.xls) with mean and integrated intensities of nucleus and cytoplasm for every channel (channel 1 – DAPI, channel 2 – marker antibody, channel 3 – protein of interest).
ExcelTemplate

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Excel

A template file for cell cycle sorting from raw measurements of ‘Nucleus Area’ and fluorescence of ‘DAPI’, ‘marker antibody’ and ‘antibody of interest’.
Excel Macro for Time estimation

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Excel

An Excel Macro-Enabled Workbook for estimating time from measurements of integrated intensities of 3 different channels.